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Journal: Drug Testing and Analysis
Article Title: Improvement of EPO Transgene Detection From Polymeric Dried Blood Spots for Antidoping Application
doi: 10.1002/dta.70008
Figure Lengend Snippet: Validation of RM‐EPO detection from TASSO DBS. A. Selectivity, sensitivity (Limit of detection) and reproducibility of detection are shown. PCR was performed using Taqman EPO assay on DNA extracted from DBS TASSO M‐20 loaded with 10 different blood samples spiked at 0–1500–2500–5000 c/mL RM‐EPO. Cq values are indicated. Examples of size of amplified products are shown for bloods spiked at 5000 c/mL. Reproducibility experiments were performed after DNA extraction from the second and third spot of the TASSO device. B. CP performed on the fourth spot of TASSO M‐20 (“B analysis”). PCR was performed using NMI CP on DNA extracted from the fourth spot (last spot) of DBS TASSO M‐20 loaded with blood samples spiked at 5000 c/mL RM‐EPO. Cq values are indicated. Examples of size of amplified products are shown.
Article Snippet: In addition to the cellulosic paper (Whatman Protein 903 Saver card) and volumetric polymer device (20‐μL Mitra, Neoteryx) that usually require finger pricks to collect blood, we evaluated another quantitative
Techniques: Biomarker Discovery, Amplification, DNA Extraction